Journal: Journal of Cancer
Article Title: Cancer Stem Cells of Diffuse Large B Cell Lymphoma Are Not Enriched in the CD45 + CD19 - cells but in the ALDH high Cells
doi: 10.7150/jca.35000
Figure Lengend Snippet: Characterization of CD45 + CD19 + and CD45 + CD19 - cells in Farage cell line. (A) the weight of tumors generated by CD45 + CD19 + cells were higher than CD45 + CD19 - cells, **p≤0.01. (B) Ki-67 analysis of CD45 + CD19 + and CD45 + CD19 - cells derived tumor xenografts by IHC (×400). (C) cells were plated at clone density (2500 cells and 1250 cells per well) and cultured for 14 days, colony formation was quantified using the fluorescent CyQUANT GR Dye. Three independent experiments were performed. ***p≤0.001. (D) the expression levels of ABCG2, Nanog, Oct4 and SOX2 were evaluated. β-actin was used as a loading control. There was no difference between the two groups.
Article Snippet: The EBV positive DLBCL cell line (Farage) was purchased from China Center for Type Culture Collection.
Techniques: Generated, Derivative Assay, Cell Culture, CyQUANT Assay, Expressing, Control